Study regarding the regenerative response after induced anemia through blood collection in mouse

Abstract

Ordinarily, animals can tolerate a loss of about 20 to 30% of the total quantity of blood without the need for transfusions or fluid therapy. Regarding mice, it is generally accepted that blood samples should be limited to 10 – 15% of total blood mass, in order to prevent the onset of anemia. For this experiment we have shown the hematological differences between the collection of 25% and 40% of total estimated blood volume. In both cases, complete blood counts were analyzed and erythropoietic response was described by means of bone marrow and spleen cytology analysis. After approximately 24 hours, both groups had increased reticulocyte counts and low hemoglobin levels. Red blood cell morphology was not altered with the exception of anisocytosis due to polychromasia.