Boar freeze-dried semen evaluation using Spermac staining

Abstract

Sperm freeze-drying is a new and alternative method to preserve male gametes in refrigeration or at room temperature. In order to protect sperm integrity special protection is required. The aim of our research was to examine the effect of vitamin C and rosmarinic acid on the spermatozoa integrity after freeze-drying the probes. We analyzed the acrosome reaction and the morphological aspects of boar spermatozoa form three different breed (Pietrain, Large White and Landrace) after rehydration. We observed that the highest percent of spermatozoa with intact acrosome and the least spermatozoa anomalies were in samples were rosmarinic acid was added. As preliminary results we could state that adding antioxidants protects spermatozoa from oxidative stress.