Callusogenetic and morphogenetic capacity of safflower explants

Abstract

For in vitro culture were optimized the conditionsof sterilization(concentration of sterilization solution and the duration of treatment), the composition of nutritive media for direct embryogenesisof seeds and callusogenesis. Two types of explants, fragments of leaves cotyledons and hypocotyls, were used for inducing in vitro callusogenesis. As callus-inducing medium served Murashige & Skoog mineral base (1962) with 4 variants of additions of growth regulators (6-Benzylaminopurine and 2,4-Dichlorophenoxyacetic acid). The higher frequency of callusogenesis was found for both typesof explants on medium with 2,4-D 0,25mg/L+ BAP0,5 mg/Land 2,4-D 0,25mg/L+ caseinhydrolysates 500 mg/L.Based on the ANOVA test it was established that the positive response is determined significantly only by the culture medium (hormonal balance) at 99.9%.