Facilitation of the rhizogenesis and caulogenesis of Epipremnum aureum by in vitro micropropagation and different Murashige-Skoog culture media formulation

Abstract

Epipremnum aureum is a houseplants cultivated in Romania for its decorative appearance given by the presence of large heart-shaped leaves. In vitro micropropagation is one of the most used laboratory techniques for the multiplication of plants under controlled conditions (humidity, temperature, photoperiod). As E. aureum is of commercial interest, in vitro micropropagation by direct organogenesis from petiole and leaf explants grown on Murashige-Skoog (MS) basal culture medium supplemented with both a carbon source (sucrose) and a gelling agent (agar-agar) is proposed. Depending on the aim, the medium was supplemented with different growth hormones. Thus, α-naphthaleneacetic acid (NAA) and 6-benzyladenine (BA) were added for rhizogenesis, and in the case of shoots regeneration the MS medium was supplemented with kinetin (Kin) and 6-benzyladenine (BA). Both root and adventitious shoot regeneration efficiency was higher from petiole explants compared to the use of leaf fragments.