Abstract:
An enhanced availability of nutrients in the culture media and the enrichment with carbon sources, nitrogen sources and micro and macro- mineral elements can ensure both qualitative and quantitative development of mycelia cultivated in vitro on solid nutrient media or in submerged cultures. The influence of diverse organic carbon sources incorporated into experimental variations of medium made with aqueous extracts from white grape marc, red grape marc, brewery waste, corn cobs, coffee grounds and fruit waste were investigated in this paper. For each stage of the technological phase in which the biological material is present, Pleurotus spp mycelia can be characterized according to their physiological state when interacting with the culture medium and the environmental parameters provided. Only uniformity, vigour, vitality, phenotypic integrity and homogeneity between samples/replicates can provide evidence of the mycelial quality.
