Abstract:
In this paper we describe an internal study for validating high
performance liquid chromatography (HPLC) method for quantification of the
biogenic amines in bananas. The evaluated features for validating the
measuring method by means of the high performance liquid chromatography
are as follows: linearity, precision, accuracy (repeatability and
reproducibility), selectivity, sensitivity (detection limit, quantification limit),
and robustness. The analyzed biogenic amines are: tryptamine, phenylethylamine, putrescine, cadaverine, histamine, serotonin, tyramine,
spermidine and spermine. The calibration curves for the biogenic amines are
linear and the values of the linearity coefficients (r2
) are as follow:
tryptamine r
2 = 0,9953, β-phenylethylamine r
2 = 0,9983, putrescin r2 =
0,9985, cadaverine r2 = 0,9985, histamine r2 = 0,9981, serotonin r2 = 0,9966,
tyramine r2 = 0,9986, spermidine r2 = 0,9986, spermine r2 = 0,9982. The
average recovery in the concentration levels 0.5 … 2 µg/ml for the banana
samples recorded the following values: tryptamine 63-93%; βphenylethylamine 80-87%; putrescine 85-99%; cadaverine 96-110%;
histamine 80-93%; serotonin 60-85%; tyramine 82-98%; spermidine 80-
105% and spermine 90-110%. The proposed method for biogenic amines
quantification in bananas by HPLC is selective. The peaks for every biogenic
amine are separated by the baseline and by the vicinity peaks. The resolution
that characterize the selectivity is greater than 1. LOD (limit of detection) for
every amine are as follows: tryptamine 0.006 µg/ml, β-phenylethylamine
0.050 µg/ml, putrescin 0.022 µg/ml, cadaverine 0.030 µg/ml, histamine 0.035
µg/ml, serotonin 0.015 µg/ml, tyramine 0.006 µg/ml, spermidine 0.005
µg/ml, spermine 0.009 µg/ml. LOQ (limit of quantitation) are as follows:
tryptamine 0.012 µg/ml, β-phenylethylamine 0.100 µg/ml, putrescin 0.044
µg/ml, cadaverine 0.060 µg/ml, histamine 0.07 µg/ml, serotonin 0.030 µg/ml,
tyramine 0.012 µg/ml, spermidine 0.01 µg/ml, spermine 0.018 µg/ml.
