Toxicity of Deltamethrin to Zebrafish Gonads Revealed by Cellular Biomarkers

Abstract

Deltamethrin is responsible for health and reproduction problems both in mammals and aquatic organisms. In this study, zebrafish adults were exposed for 15 days to 0.25, 0.5, 1, and 2 μg L−1 non-lethal concentrations of deltamethrin, knowing that is used worldwide on agricultural crops. We investigated the chronic effects of deltamethrin on gonads by histopathological examination, immunohistochemistry, and immunofluorescence using biomarkers for apoptosis (anti-p53, anti-H2A.XS139ph antibodies, and TUNEL assay), oxidative stress (anti-Cox4i1 antibody) and proliferation (anti-PCNA antibody). Among the histopathological changes, the apoptotic response was elevated in ovary and testis of deltamethrin exposed groups as it was seen in the IHC and IF for p53, H2A.XS139ph, and confirmed by TUNEL assay. These were observed in the case of all studied concentrations compared with the control group. Thereby, the gonadal tissue exhibited an up-regulated activity of this cell-death signaling markers, while the proliferation marker (PCNA) increased in the ovary due to its presence not only in primary growth and cortical-alveolar stage follicles but also in atretic follicles, meanwhile decreased notably in the testis. Cox4i1, a mitochondrial marker, decreased both in ovary and testis during deltamethrin treatment, probably inhibited by the overproduction of the free radicals after pesticide exposure.