Detection of Orf virus and papillomavirus out of samples from goats and cattle which were gathered by multiplex PCR Romania

Abstract

The lesions which appear on the skin and are caused by parapoxvirus, herpesviruses and papillomavirus, depending on they’re gravity, can produce large economic losses. The identification of viral species with different laboratory methods can facilitate making a correct diagnostic in identifying the infectious entity. The most common encountered injuries , especially on goats and kids, have an oral location, breasts, eyes and genital and podal. On the other side on cattles infected with papillomavirus the lesions are multiple and spread all over the body. To identify the etiologic agent from goats and cattle with skin lesions, tissue samples were collected and scabs. The virus identification was made with molecural biology techniques. The PCR method revealed the viral DNA, thus identifying a portion of the B2L preserved gene which is then amplified using primers PPP1-PPP4. For papillomavirus detection, primers FAP59 and FAP 64 were used to conserve the L1 human gene, primers that can help detect other types of papillomavirus from other species. The PCR results showed that lesions from goats were made by Orf virus, a virus that belongs to the papillomavirus gene, as for cattles the bovine papillomavirus. Studies have shown that PCR is a rapid laboratory method which can be used to identify the viral agent that causes severe skin lesions.