Abstract:
The lesions which appear on
the skin and are caused by parapoxvirus,
herpesviruses and papillomavirus,
depending on they’re gravity, can produce
large economic losses. The identification of
viral species with different laboratory
methods can facilitate making a correct
diagnostic in identifying the infectious
entity. The most common encountered
injuries , especially on goats and kids, have
an oral location, breasts, eyes and genital
and podal. On the other side on cattles
infected with papillomavirus the lesions are
multiple and spread all over the body. To
identify the etiologic agent from goats and
cattle with skin lesions, tissue samples were
collected and scabs. The virus identification
was made with molecural biology
techniques. The PCR method revealed the
viral DNA, thus identifying a portion of the
B2L preserved gene which is then amplified
using primers PPP1-PPP4. For
papillomavirus detection, primers FAP59
and FAP 64 were used to conserve the L1
human gene, primers that can help detect
other types of papillomavirus from other
species. The PCR results showed that
lesions from goats were made by Orf virus,
a virus that belongs to the papillomavirus
gene, as for cattles the bovine
papillomavirus. Studies have shown that
PCR is a rapid laboratory method which can
be used to identify the viral agent that
causes severe skin lesions.
