The dynamics of total polyphenols, flavonoids and antioxidant activity of beepollen collected from Moldavia area, Romania

Abstract

The analysis sought to study the determination in timp and different conditions (temperature and light) of total polyphenols, flavones, total flavonoids and antioxidant activity from beepollen obtained with pollen colectors from Moldavia, Romania. Two methods vave been used, consisting in different concentrations of the methanolic solvent used. Analyses were performed on fresh pollen, stored for one and two weeks; at 4°C and 22°C; presence or absence of natural light. For every determination, five repetitions were made. There were fluctuations in the amounts of compounds, depending on the storage conditions and on the solvent used. The total polyphenols quantity ranged from 24.73 mg GAE/g to 28.8 mg GAE/g, when the methanolic solvent concentration was 96 % and from 21.67 mg GAE/g to 26.50 mg GAE/g, when the methanolic solvent concentration was 70 %. The flavones detected ranged from 7.23 mg QE/g to 8.93 mg QE/g (methanolic solvent 96%), and from 5.45 mg QE/g to 6.35 mg QE/g (methanolic solvent 70%). Total flavonoids have recorded values between 12.6 mg QE/g and 20.44 mg QE/g for the m.s. of 96%, and between 14.6 mg QE/g and 17.47 mg QE/g for the m.s. of 70%. The detected antiradical activity ranged from 22.68% to 29.78% inhibition (m.s. 96%), respectively from 21.31% to 25.55 % inhibition (m.s. 70%).