Abstract:
The analysis sought to study
the determination in timp and different
conditions (temperature and light) of total
polyphenols, flavones, total flavonoids and
antioxidant activity from beepollen obtained
with pollen colectors from Moldavia,
Romania. Two methods vave been used,
consisting in different concentrations of the
methanolic solvent used. Analyses were
performed on fresh pollen, stored for one
and two weeks; at 4°C and 22°C; presence
or absence of natural light. For every
determination, five repetitions were made.
There were fluctuations in the amounts of
compounds, depending on the storage
conditions and on the solvent used. The total
polyphenols quantity ranged from 24.73 mg
GAE/g to 28.8 mg GAE/g, when the
methanolic solvent concentration was 96 %
and from 21.67 mg GAE/g to 26.50 mg
GAE/g, when the methanolic solvent
concentration was 70 %. The flavones
detected ranged from 7.23 mg QE/g to 8.93
mg QE/g (methanolic solvent 96%), and
from 5.45 mg QE/g to 6.35 mg QE/g
(methanolic solvent 70%). Total flavonoids
have recorded values between 12.6 mg
QE/g and 20.44 mg QE/g for the m.s. of
96%, and between 14.6 mg QE/g and 17.47
mg QE/g for the m.s. of 70%. The detected
antiradical activity ranged from 22.68% to
29.78% inhibition (m.s. 96%), respectively
from 21.31% to 25.55 % inhibition (m.s.
70%).
