Biochemical and microbiological study concerning identification role of hydrolase activities from Trichoderma harzianum and Trichoderma koningiiin pathogenic fungus F. oxysporum inhibition

Abstract

The genus Trichoderma are a very large group of microorganisms that play a significant role in plant protection. Several Trichoderma spp. like Trichoderma harzianum and Trichoderma koningii strongly affected plants by stimulating plant growth, and protecting plants from fungal and bacterial pathogens such as Fusarium oxysporum. They are used as a biological plant protection as bio fungicides.Members of the Trichoderma spp. are also utilized in different branches of industry -principally in the areas of enzymes, antibiotics, and other metabolites. In this study we focus in the effect of T. harzianum strain ICCF 417 and T. koningiistrain ICCF 418 on F. oxysporum(ZUM 2407) by microbiologic and enzymatic tests. Where the results of fungal growth speed of the malt medium showed that the fungus T. koningiiwas the fastest in growing, followed by T. harzianumand F. oxysporumafter 72 hours of culture. While the degree of antagonism was 1 according to Bell scale in petri dish on the PDA medium the ability of fungi T. harzianum and T. koningiito overcome on fungus F. oxysporum. The results of the study showed the susceptibility of bio-fungi on production of an enzyme FPase was 32.3 % in T. harzianum comparative toT. koningii after 14 days of fermentation, the amylase was 84.5% in T. harzianum comparative toT. koningii while the CMCase was 36.6 % inT. harzianum comparative toT. koningii.Our results showed that hydrolase activities studied in this experiment play an important role in pathogenic fungus F. oxysporum inhibition and the degree of effect is different.