Study of the anti-pregnancy - associated glycoprotein (anti-PAG) resulted from antigen PAG immunization, as prospective early pregnancy detector in animals

Abstract

Blastocyst protein named pregnancy-associated glycoprotein (PAG) has been isolated from pregnant dairy cow serum. PAG yielded from previous research issued from pregnant animals, wherein was specifically produced. This study is an initial research to use PAG as an antigen to be injected to rabbit in order to produce the corresponding antibody. The objective of the research was to study the character of anti-pregnancy-associated glycoprotein (Anti-PAG), as a result of immunization of PAG and to learn its specification reaction. The PAG isolate had bovine origin (molecular weight-MW of 67.34 kDa) and issued from previous research. Injection of PAG isolate could stimulate the production of Anti-PAG as an immunization response. Immunization was done by double booster inoculation. Anti-PAG derived from immunization was characterized via antibody titer value using ELISA technique. Specificity test of Anti-PAG was carried on by Western Blot technique. Results revealed that injection of PAG isolate to the rabbit stimulates anti-PAG production. Concentration of Anti-PAG was 1.192 μg with titer of 1.044. Anti-PAG derived from rabbits recognized Standard PAG antigen (bovine PAG MDBiomed cat. 101-7963-13- 3) and PAG isolate derived from previous research. The reaction of antigen antibody is the basic structure of creating a gestation detection kit. Anti-PAG is expected to be the molecular marker in developing a gestation detection kit in the near future.