Abstract:
Blastocyst protein named
pregnancy-associated glycoprotein (PAG)
has been isolated from pregnant dairy cow
serum. PAG yielded from previous research
issued from pregnant animals, wherein was
specifically produced. This study is an
initial research to use PAG as an antigen to
be injected to rabbit in order to produce the
corresponding antibody. The objective of
the research was to study the character of
anti-pregnancy-associated glycoprotein
(Anti-PAG), as a result of immunization of
PAG and to learn its specification reaction.
The PAG isolate had bovine origin
(molecular weight-MW of 67.34 kDa) and
issued from previous research. Injection of
PAG isolate could stimulate the production
of Anti-PAG as an immunization response.
Immunization was done by double booster
inoculation. Anti-PAG derived from
immunization was characterized via
antibody titer value using ELISA technique.
Specificity test of Anti-PAG was carried on
by Western Blot technique. Results revealed
that injection of PAG isolate to the rabbit
stimulates anti-PAG production.
Concentration of Anti-PAG was 1.192 μg
with titer of 1.044. Anti-PAG derived from
rabbits recognized Standard PAG antigen
(bovine PAG MDBiomed cat. 101-7963-13-
3) and PAG isolate derived from previous
research. The reaction of antigen antibody is
the basic structure of creating a gestation
detection kit. Anti-PAG is expected to be
the molecular marker in developing a
gestation detection kit in the near future.
