Abstract:
Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) has
become a cornerstone technology in modern microbiology. Its implementation within veterinary diagnostics,
specifically through the Bruker MALDI Biotyper Sirius One system at the ROVETEMERG platform, provides
a rapid, high-throughput, and cost-effective method for identifying a wide spectrum of bacterial and fungal
pathogens. This article delineates the analytical workflow—from sample preparation and matrix cocrystallization
to spectral acquisition and bioinformatic interpretation—and evaluates its performance in a
veterinary context. The technique's reliance on conserved ribosomal protein profiles generates species-specific
molecular fingerprints, enabling reliable taxonomic identification. The integration of proprietary Bruker
libraries with public health databases like CDC MicrobeNet significantly enhances the detection of rare,
emerging, and zoonotic pathogens. Furthermore, an analysis of identification data from 2024-2025 reveals a
microbial prevalence profile dominated by Escherichia coli (3249 identifications), followed by other
significant veterinary pathogens such as Proteus mirabilis and Staphylococcus aureus. The platform's
demonstrated efficacy in routine diagnostics, outbreak investigation, and antimicrobial stewardship
underscores its pivotal role in advancing veterinary medicine and strengthening One Health surveillance
systems.
