Izolarea substanţelor active cu caracter antifungic din Paeonia suffruticos respectiv Hedera helix

Abstract

The presence of antifungal active compounds in Paeonia suffruticosa and Hedera helix extracts was detected with TLC bio tests (HOMANS A.L. & FUCHS A., 1970 modified) and HPLC. So 25 µl of acetone extract (20 g FW in 100ml acetone 50%) from both plant species was applied on TLC aluminium plates (silica gel 60 F254; thickness 0,2 mm; Merck, Darmstadt) and processed with an solvent mixture cu tertiary butylmethylester (TBME) isooctane (7:3, v/v). After solvent evaporation (cca. 3 h), the plates were covered with malt- agar medium 1% and than an inoculation with Cladosporium cucumerinum spores was done. The TLC plates was incubated for 4-5 days in wheat chamber at 25°C, the inhibition areas was visible due the black mycelia of Cladosporium cucumerinum.