Abstract:
Choosing this species as the investigation material is motivated by a
small number of existed studies, and the dates that will be obtained can be
used to improve plants. The investigation of cell division is a permanently
preoccupation, this process having a major importance in development of the
individual biological systems. In this approach we propose to analyse by
citogenetic and biochemic way, Thuja orientalis L. from Cupressaceae
family. Thus, we treated Thuja orientalis L. seeds with solutions of ascorbic
acid (E300) and citric acid (E330) in three different concentrations: 0.1 %,
0.25 % and 0.5 %. The results we obtained were colligated with the etalon.
We observed the stimulator or inhibitor effect of ascorbic acid and citric acid
to the mitotic division and estimated the aberrations appearance. After
calculation, we observed that citric acid has a strong effect to the roots of
Thuja orientalis L. establishing the increase of aberrations in cells. Between
aberrations, the simples and multiples bridges are predominant. This
explained that the used substances blocked the migrations of chromosomes to
the poles of the division axle. The small number of aberrant ana-telophases
after the treatment with ascorbic acid can be determinated by a strong
antioxidant effect of this substances. The mitotic index increases at seeds
treated with citric acid, which indicates that this substance stimulates the
cells division. The biochemicals investigations was based on the estimation of
respiration enzymes activity (superoxid dismutase, peroxidase and catalase).
The enzymes activity is inhibited by high concentrations, comparative the
etalon. Behind the biochemicals analyses, we observed that superoxid
dismutase(SOD) and catalase are inhibited of high concentrations. By other
way, the peroxidase activity is stimulate at both treatments. In conclusion,
this two substances has influence to the reactive species of oxygen and this
reflect the cell stress state.
