Abstract:
The paper presents the influence of heavy metals upon the mitotic
division of Papaver somniferum L. specie. The treatment with salts of heavy
metals determined the lessening of the mitotic index and the appearance of
chromosome modifications whose rate was different. This rate depends upon
the concentration level and the period of action for the above mentioned
substances in this experiment.
The salts of heavy metals used in this experiment are: CuSO4, CoSO4,
Zn (NO3)2. Copper, cobalt and zinc are known as heavy metals, which make
part of the category of essential oligominerals.
The treatments were applied for four hours, at Papaver somniferum
L., in different concentrations. The concentrations we used were 1% and 5%
for each substance.
After the germination of Papaver somniferum seeds, in laboratory
conditions at 22°C, the genetically displays were made. For each display, 40
microscopically fields were studied and there were counted all cells from
each field in different division phases and chromosomal aberrations which
appeared after the treatment.
In order to study the chromosomal frequency aberrations in ana –
telophases, there can be observed the frequency of division cells and there
were counted the ana – thelophases which presented chromosomal
aberrations.
After the examination of the displays we observed the appearance of
various chromosomal aberrations in ana – thelophases, like: micronuclei,
retardate chromosomes, tripolar ana – telophases, bridges, fragments.