Abstract:
At the end of January 2014, it was created the Laboratory for assisted reproduction in the Horia Cernescu Research Unit
(IVF-CLCHC). Our immediate concern was to put together, from the scratch, all especially techniques which are usual
in such a lab. The Assisted reproductive techniques we have put together, regarding the degree of difficulty and all the
necessary steps were IVP (in vitro production), IVF (in vitro fertilization), ICSI – intracytoplasmic sperm injection and
NT (nuclear transfer). PCR, rt-PCR, or qPCR were used for the assessment of the gene expression levels in all the selected
genes in gametes, zygotes, or embryos. Despite all inherent difficulties, we were able to put together all the forementioned
techniques and obtain satisfactory results. We were able to increase the difficulty degree of used techniques gradually,
gaining skills and good experience. The obtained results, in some cases represented the starting point for PhD thesis
research. The propper number of personnel working in the lab ensures a continous flow of work and appointed research
themes. The creation and existence of the Laboratory for assisted reproduction in the Horia Cernescu Research Unit (IVFCLCHC)
does represent a success, opening a lot of opportunities for those interested in the field of assisted reproduction
and also for our University itself.
