Inclusion complex of thiotriazinone with α-cyclodextrinraman spectroscopy, DSC, preliminary antimicrobial and antifungal study

Abstract

Thiotriazinone (TTZ) is used as an intermediate of cephalosporin, but very few things are known about its activity. The purpose of this study was to form a complex of Thiotriazinone-α-cyclodextrine and to investigate its antimicrobial activity, in order to use this ”smart drug” in animal diseases therapy. The complex was made by liophlization at a molar ratio TTZ-αCD=1:1. Characterization was made by Raman spectroscopy and DSC. Antimicrobial activity was tested in vitro on Gram-positive bacteria (Staphylococcus aureus ATCC 25923, Sarcina lutea ATCC 9341), Gram-negative bacteria (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC), and fungus (Candida albicans ATCC 10231, Candida glabrata ATCC MYA 2950, Candida parapsilosis ATCC 22019). The inclusion complex showed better antibacterial effect for S. aureus and S. lutea versus thiotriazinone. It may be noted that the compounds tested showed no antibacterial activity for E. coli and Pseudomonas aeruginosa. Although the tested compounds show antifungal activity against all fungal organisms, a more pronounced action was observed especially against C. glabrata.