Abstract:
In Europe, including Romania, vector-borne illnesses are widespread. In addition to endangering the
health of people and animals, these diseases have a
significant impact on the global economy. From October 2022 to September 2023, 25 canine blood samples
and 21 human serums were analysed for Anaplasma
phagocytophilum and Anaplasma platys, and Borrelia
spp. + A. phagocytophilum respectively. Canine samples were analysed using three methods: cytological
blood smear, serologic, and molecular, and for human
serum, we used two microblot assays for confirmation: Borrelia spp. + A. phagocytophilum IgG antibodies and Borrelia spp. + A. phagocytophilum IgM
antibodies. Intracytoplasmic morula was observed in
neutrophils in 8/25 (32%) dogs, and Anaplasma antibodies were detected in 11/25 (44%) canine serum. In
qRT-PCR, 4/25 (16%) dogs were positive for A. phagocytophilum. According to the microblot assay results, 38.1% of the serum samples were positive for
both species - Borrelia and Anaplasma, and 33.4%
were positive only for Borrelia. The aim of this study
was to evaluate the seroprevalence of human granulocytic anaplasmosis cases as well as the prevalence of
canine granulocytic anaplasmosis, including the possible co-infections with other pathogens, using several diagnostic methods.
