Histochemical researches regarding abnormalies within the development of walnut (Juglans regia L.) male reproductive system

Abstract

During the development of walnut (Juglans regia L.) male sexual system the most frequently noticed anomalies there are conditioned by low temperatures (-10 - -150C) in the meiotic period (the end of March - first half of April months). As a rule in the exterior part of inflorescences of protandrous as well as of some protoginous genotypes microsporogenesis is lead rapidly. In the microspores mother cells (MMC) appear big vacuoles, pushing slowly hypertrophied nucleus to tapetal tissue. There are observed typical phenomenon of cytomixis and picnosis with passage of fragments of nucleus and nucleols from one cell to another. Tapetal tissue is affected too, but there could be founded some sectors with normal development. Vacuolization of tapetal cells is very expressed adjacency MMC , citoplasm and nucleus are moved to parietal layer. Intensity of NA, total proteins and polysaccharides test reactions there are low. Reaction of starch test is null, and for lipids –remarkably low. Anomalies there are observed more frequently for protandrous as well as protoginous genotypes with accelerated rhythm of male flower development.