Lipid fractions analysis in pork meat by highperformance thin layer chromatography

Abstract

High performance thin layer chromatography is a fast and eficient method for the separation of complex mixtures. The HPTLC analysis of lipid fractions on silica gel plates used for extraction a solvent mixture consisting of hexane, chloroform and ethanol and as reagent color a mix of copper sulphate and phosphoric acid. The resulting chromatogram presented a variable baseline and five corresponding peaks to the following lipid fractions: cholesterol esters- triacylglicerols, free fatty acids, cholesterol, phospholipids, mono- and diacylglicerols. Interpretation of the HPTLC analysis results can provide information about both the (%) composition of the major lipid fractions and about the freshness of the samples analyzed (meat of Landrace pork raised with industrial diet or in tradional farm versus meat of Vietnamese pork raised in tradional farm).