Protein content stability of triticale genotypes under multiple environments using AMMI analysis

Abstract

Triticale is an important source of protein in animal nutrition, both as grain and silage. Protein is a quality criterion that is strongly affected by the environment and genetic factors. The objectives of this study were to assess genotype–environment (G-E) interactions and determine and compare stable genotypes to recommend for further evaluation. The protein content of 12 genotypes obtained from 7 environments were evaluated using additive main effect and multiplicative interaction (AMMI) analysis in the 2014–2015 and 2015–2016 growing seasons in 4 locations in Turkey. The variance of AMMI analysis based on protein content showed a significant effect of environment, genotype and G–E interaction, with a 93.0, 2.3 and 4.5% total variation, respectively. Partitioning of the total sum of squares showed that the effect of environment was a predominant source of variation, followed by the G–E interaction and genotype effect. AMMI analysis showed that the first principal component was highly significant at P < 0.01. The mean grain protein content varied from 14.9 to 16.2% among the genotypes and from 10.9 to 18.8% among the environments. AMMI analysis indicated that G3, G12 and G1 were quite promising genotypes. G8, which was used as a variety candidate in this research, was quite stable but had a low protein value. In addition, G3, G11 and G12 had a higher protein content than the standard varieties evaluated in this research. G3 had both a high protein content and stable genotype; therefore, it can be recommended to release as a candidate. As AMMI analysis indicated, E1 and E7 environments were especially suitable for protein studies in triticale, while E4, E5 and E6 showed poor results and were not suitable environments.