Abstract:
The antioxidant coenzyme Q10 can influence the expression of genes involved in apoptosis and energy metabolism of
oocytes and quercetin can improve oocyte maturation and early embryonic development. In this study, the gene
expression of GATA6 and NANOG in rabbit embryos was assessed using the qRT-PCR reaction. The groups were:
group A- control group (no treatment added), group B (hormonal treatment of superovulation, which included the
administration of PMSG and hCG), group C (administration of quercitin) and group D (administration of Coenzyme
Q10). Our results show that the expression of the two genes was different depending on both the stage of embryonic
development and the treatment administered. The highest values of gene expression for GATA6 and NANOG were
obtained in groups 2, 4, 7, 8 and 9, corresponding to morula and blastocyst stages. In addition to the fact that NANOG
and GATA6 are factors that are involved in early embryonic development, we believe that the administration of
extrapituitary gonadotropins and antioxidants contributed to the increase in gene expression.
