Rabbit embryo generation through various protocols

Abstract

In rabbit ovulation is induced by mating stimulus. The length of ovulation influences the embryo developmental stages and their localization in various segments of genital tract during first 72-84 hours post mating. The study has compared results obtained in 32 female from four groups: group 1 (control, 8 females, simple mated), group 2 (8 females treated with 180 IU of HCG), group 3 (8 females, treated with 30mg/kg quercitine/day P.O. for 20 days) and group 4 (8 females receiving CoQ10 10mg/kg/day P.O. for 20 days). The corpora lutea (CL) and total number of embryos (TNE) in uterine horns was influenced by treatments (F=5.129 at p=0.006, respectively F=3.59 at p=0.026) and were higher in group 2 and 3. TNE was positively correlated with total number of CL (r=+0.484, p=0.003), with the number of embryos flushed form uterine horns (r=+0.889, p=0.000) and oviducts ((r=+0.921, p=0.003), with weight of uterus (r=+0.621, p=0.000), and negatively correlated with ovaries weights (r=-0.730, p=0.000), In conclusion, regarding the total number of CL and number of obtained embryos, the most efficient treatment was the hormonal one (applied for group 2), data characterizing the success of generation.