Effect of long-term exposure to non-thermal plasma activated water on methemoglobin in mice

Abstract

Non-thermal plasma activated water (PAW) is described as a potent antimicrobial agent, but although it has numerous bio-medical applications, there is a lack of toxicity studies in living organisms. Thus, as a main objective, we aimed to evaluate the in vivo methemoglobin sizing potential of non-thermal plasma activated water (PAW) in CD-1 mice. The device used in our experiment is based on the GlidArc principle, with the advantage of adjusting the values of the current in the circuit on account of a special power supply, which works with magnetic scattering fluxes. A daily volume of 300 ml of PAW was prepared daily with this reactor with the following physico-chemical parameters: conductivity 446 ± 25 μS / cm, pH 2.78 ± 0.12, ORP + 1.06 V, NO2-192 ± 10 mg / L, NO3-1550 ± 95 mg / L, H2O2 2.6 ± 0.12 mg / L, O3 1.08 ± 0.07 mg / L, peroxynitrite - ONOO-. After analysis and interpretation of the data, it was found that methemoglobinemia did not differ significantly in the groups treated with PAW (p <0.05) compared to the control group (p = 0.8076). Thus, long-term consumption of PAW has no detrimental effects on the health status of CD-1 mice.