Response to poliovulatory (POV) treatment, by ultrasound in Suffolk breed

Abstract

This study was done on a batch of Suffolk beef sheep, acclimatized in Romania.. The success of an ET protocol in sheep depends on many factors, but in the end, what matters is the number of embryos obtained. Embryo recovery (recovery rate), after poliovulation (POV), is an essential step in ET. The aim of our experiment was to observe the ovarian reaction (follicles –F, and corpora lutea-CL) to the treatment of Suffolk sheep polyovulation, The aim of our experiment was to observe the ovarian reaction to the treatment of Suffolk sheep POV. A number of 3 Suffolk sheep were poliovulated at the reproductive season, using P4-FSH-PGF protocol. The POV method was based on the administration of sponges with intravaginal progesterone 12 days, followed by 500 IU FSH: LHp in decreasing doses in the last 4 days, and a PGF on day 11. The poliovulatory ovarian response was monitored by transectal ultrasound, before estrus was detected, and on the day 7. The ovulatory response following POV treatment was assessed by CL counting. In two out of three sheep, CL was identified, despite the fact that they all had follicular growth, in sheep no. 2, no dehiscence occurred. The total number of formations observed was 26 CL. The distribution of CL between the two right and left ovaries, in the three cases examined was thus 8 CL on the right ovary and 7 CL on the left (in sheep 1), no CL in sheep 2 and 6/5 in sheep no. 3. The POV response to FSH in the Suffolk breed was an average of 8.6 F/sheep of the developed follicles. Our goal was in fact to follow the ovarian dynamics in these meat breeds, in order to apply the best treatment schemes, for successfully apply embryo transfer.