ALSERepository of Iași University of Life Sciences, ROMANIA

In vitro bovine embryos evaluation based on OCT4, SOX2, IGF1R and IGF2R expression level

Show simple item record

dc.contributor.author Marc Zarcula, Simona
dc.contributor.author Mircu, Călin
dc.contributor.author Tulcan, Camelia
dc.contributor.author Bonca, Gheorghe
dc.contributor.author Huțu, Ioan
dc.contributor.author Otavă, Gabriel
dc.contributor.author Torda, Iuliu
dc.contributor.author Georgescu, Ovidiu-Ionuț
dc.contributor.author Andonii, Cristina
dc.contributor.author Lungu, Bianca-Cornelia
dc.contributor.author Boldura, Oana-Maria
dc.date.accessioned 2021-02-24T08:47:45Z
dc.date.available 2021-02-24T08:47:45Z
dc.date.issued 2020
dc.identifier.citation Marc, Simona, Călin Mircu, Camelia Tulcan, Gh. Bonca, Ioan Huțu, Gabriel Otavă, Iuliu Torda et al. 2020. "In vitro bovine embryos evaluation based on OCT4, SOX2, IGF1R and IGF2R expression level". Lucrări Științifice USAMV - Iași Seria Medicină Veterinară 63 (2): 151-158. en_US
dc.identifier.uri https://repository.iuls.ro/xmlui/handle/20.500.12811/280
dc.description.abstract In vitro production of bovine embryos comprises a lot of factors that can influence the successful of this technique, oxidative stress being one of them. These factors can influence the evolution of important development processes such as the maternal to zygotic transition and the embryonic genome activation. Adding antioxidants to in vitro culture media exerts the key role to reduce the effects of reactive oxidative species produced during assisted reproduction technique, influencing in a positive way also the early embryonic developement. The objective of this study was to determine the effect of antioxidant rosmarinic acid (105 μM), added to in vitro bovine oocytes maturation media, on the quality of embryo produced based on gene expression level of OCT4, SOX2, IGF1R and IGF2R. For this purpose, we used 35 bovine ovaries taken from slougtherhouse from which we obtain 202 cumulus-oocyte-complexes and 127 of them were maturated in vitro based on morphological aspects. The cumulus-oocyte-complexes were divided in two groups: control (M1, M2, M3) and with acid rozmarinic (AR1, AR2 and AR3). The levels of OCT4, SOX2, IGF1R and IGF2R were the highest in group AR1, embryos obtain from oocytes class I supplemented with rozmarinic acid, where OCT4 expression was 4.08, SOX2 was 27.66, IGF1R and IGF2R were 53.44 and 25.10. en_US
dc.language.iso en en_US
dc.publisher ”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași en_US
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject cumulus-oocyte-complexes en_US
dc.subject antioxidants en_US
dc.subject gene expression en_US
dc.subject bovine en_US
dc.title In vitro bovine embryos evaluation based on OCT4, SOX2, IGF1R and IGF2R expression level en_US
dc.type Article en_US
dc.author.affiliation S. Marc, C. Mircu, C. Tulcan, Gh. Bonca, I. Huțu, G. Otavă, I. Torda, O. Georgescu, C. Andonii, B. Lungu, O. M. Boldura, University of Agricultural Sciences and Veterinary Medicine of Banat “The King Michael I of Romania”, Faculty of Veterinary Medicine, Timisoara, Romania
dc.publicationName Lucrări Științifice USAMV - Iași, Seria Medicină Veterinară
dc.volume 63
dc.issue 2
dc.publicationDate 2020
dc.startingPage 151
dc.endingPage 158
dc.identifier.eissn 2393-4603


Files in this item

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)