Turbidimetric determination of raw fat in crop seeds

Abstract

A variant of a turbidimetric method of raw fat determination from seed, which proved to be simple, fast, and accurate, was described in this paper. The method was based on the extraction of lipids from 20 mg samples into acetone followed by their treatment with 1.5% solution of sulfosalicylic acid and spectral measurement at 440 nm against a blank of the reagents. Suitable volumes of acetone extracts should be taken for oily seed samples such as flax, sunflower, or soybean. A standard curve was made with raw fat extracted from the species being analysed. For analysing a large number of samples, the standardization of the turbidimetric method with a few representative Soxhlet values was recommended. Possible interferences, as well as the real results obtained within large scale analyses ,are also shown.