Serological evaluation of canine coronavirus antibodies

Abstract

Canine enteric coronavirus (CECoV) is an infectious disease, affecting dogs and being responsible for the occurrence of diarrhoea with various degrees of severity in puppies. Research has covered two main directions: the establishment of canine coronavirus carriers among healthy animals and those with digestive troubles (diarrhoea, vomiting and weight loss), and the assessment, after vaccination with canine anticoronavirus vaccine, of specific antibody titer type IgG and IgM. The values of optical density, determined by indirect ELISA for anticoronavirus antibodies type IgM, have varied from 93 to 1004, showing the specific immune response after infection and the establishment of canine coronavirus carriers and disposers. The values of optical density, determined by indirect ELISA for anticoronavirus antibodies type IgG, have varied after 14 days, from 89 to 556, while after 21 days, from 312 to 723. In case of anticoronavirus antibodies type IgM, after 14 days, the same values varied from 43 to 267, while after 21 days, from 45 to 253, showing the specific immune response after vaccine. The obtained results allowed us to assess the importance of knowing the carrier and removal status of canine coronavirus, as a source of infection for other animals, not necessarily belonging to the same breeds. These could develop very severe diseases, even lethal ones.