Aspects regarding immune prophylaxy in enzootic bovine ringworm

Abstract

The aim of this study was the isolation and identification of Trichophyton strains and species on various selective culture media (Sabouraud dextrose agar and Dermatophyte Test Medium - DTM); morphological and cultural characterization of Trichophyton isolated strains; calves immune response consecutive to the administration of the Trichoben vaccine. The experiment was carried out during October 2008 – February 2009 on a group of 20 animals: 15 animals with typical ringworm lesions and five healthy control calves. For the isolation and the morpho-cultural characterization of Trichophyton, samples consisting of crust, squames and hairs were collected and examined by using enlightening solutions and were inseminated on special culture medium for dermatophytes. Ringworm prevention and control were a difficult problem. Various therapeutic schedules gave inconstant results; thus, we took into account the implementation of systemic vaccination programs. We have used the Trichoben vaccine prepared with a nonvirulent strain of Trichophyton verrucosum (min. 2.5 x 106 CFU), which was lyophilized. Preventively, the dose was of 2.5 ml in three week –three month old calves, while for older than three month calves, it was of 5 ml, repeated 10-14 days later for both age categories. For the therapeutic purpose, doses were double. Good results were observed since the first administration, while after 28 days, healed placards and lesion diminution, crust thinning and detaching were noticed. Vaccination efficiency for therapeutic purpose was of approximately 95%.