Abstract:
To evaluate the response of
proline and soluble sugars content, catalase
(CAT) and ascorbate peroxidase (APX)
activity in wheat leaves to different
irrigation regimes at two growth stages, a
greenhouse factorial experiment was
conducted in a completely randomized
design (CRD) with three replications. The
factors consisted of four different irrigation
regimes as 100% (I0), 75% (I1), 50% (I2) and
25% (I3) of field capacity and growth stages:
50% emergence to booting stage (GS1) and
booting stage to physiological maturity
(GS2). Fresh leaf tissues were used to
determine proline and soluble sugars
content, CAT and APX activity. According
to the results, irrigation regimes had
significant effect on proline content, soluble
sugars and APX activity, but no significant
differences were detected among irrigation
regimes for catalase (CAT) activity. Limited
irrigation increased proline concentration
and total soluble sugars in leaves. None of
studied traits were affected by growth
stages. Interaction between irrigation
regimes and growth stages was not
significant for all studied traits. Results
indicated that the highest proline content (12
μmol/g DW), total soluble sugars (49 mg/g
DW) and APX activity (42 μmol min-1 g-1
FW) were related to irrigation at 25% of
field capacity (I3). It was concluded that
proline and soluble sugar levels were
increased in wheat leaves under deficit
irrigation regimes.
