Abstract:
Grape pomace (GP), by-product of wine making, contains a large
part of antioxidant polyphenols originating from the input material, the grapes.
High production volumes, environmental impact and nutritional content of
grape pomace makes it an important subject for careful valorisation. In order to
valorize it, it is necessary to determine its chemical composition in bioactive
molecules. In this study the anthocyanins were determined by LC-MS from a
dried red grape pomace from Valea Călugărească, Romania. Based on their
retention times, UV-VIS and MS spectra using standard compounds one
anthocyan and three anthocyanidins were identified and quantified from GP
acetone extract: peonidin 3-O-glucoside, delphinidin, cyanidin and malvidin.
The most abundant anthocyanidin is delphinidin (23.93 ±0.12 μg/mL acetone
extract) followed by malvidin (10.01 ±0.06 μg/mL acetone extract), the
anthocyan peonidin 3-O-glucoside (4.78 ±0.04 μg/mL acetone extract) and
cyanidin (2.63 ±0.06 μg/mL acetone extract). The results show that GP contains
anthocyans and anthocyanidins, which possess anti-inflammatory and anti-
carcinogenic activity, cardiovascular disease prevention, obesity control, and
diabetes alleviation properties, all of which are more or less associated with
their potent antioxidant property.
