Polimorphism screening using a vntr molecular marker system in romanian resident bison individuals

Abstract

Genomic DNA isolated from the blood sampled from 14 individuals of Bison bonasus was used in order to obtain a molecular fingerprint and based on that, to obtain a dendrogram exhibiting the phylogentic relationship among those individuals. By using the PCR technique and an VNTR ( Variabile Number of Tandem Repeats molecular markers system: ISSR (Inter simple sequence repeats) was used in this study for establish set of DNA fingerprints. Based on molecular data a common binary matrix was developed. By using DendroUPGMA software, based on Jaccard coefficient the similarity indices and the genetic distances were calculated. Based on those data set a dendrogram containig the phylogenetic relationship among bison exemplars, was generated. By analyzing the molecular data it was observed that the degree of polymorphism was either absent or very low especially in this case, our recorded data suggest that among Romanian resident Bison exemplars, there is a high level of genetic similarity meaning that the individuals are most probably close blood relatives.