Quality evaluation of freeze-dried ammodytes ammodytes venom by CGE on-a chip with laser induced fluorescence detection

Abstract

Our study represents the first attempt to develop a protocol for evaluating the quality freeze- dried venom by using current proteomics analysis techniques The research carried out represents the initial step in the validation of the method for protein analysis by capillary electrophoresis gel with laser induced fluorescence detection , being our first attempt in determine the impairment of protein fractions during long storage of the freeze-dried venom . Freeze-drying was used as a method of advanced dehydration Ammodytes ammodytes venom and the yield of these procedure went up to 25.92 %. On a long-term storage at -20 ° C freeze-dried venom of the factions protein degradation occurs , the most affected being that with the molecular weight of approx . 28 and 37 kDa , respectively identified as Vammin and phospholipase A2 , as demonstrated on the one hand, by decreasing the % of these fractions and on the other hand, by increasing the % of protein fractions obtained by degradation.