Relationship between salt tolerance related physiological traits and protein markers in soybean cultivars (Glycine max L.)

Abstract

This study was conducted to evaluate the salinity tolerance in seedling stage of soybean (Glycine max L.). Factorial experiment was done based on randomized complete block design with three replicates. 17 soybean genotypes were used in three salinity stress levels (consisting of control, 75 mM and 150 mM NaCl stress). The experiment was carried out in a greenhouse condition and proline, sodium, potassium, and chlorophyll a, chlorophyll b, chlorophyll a/b and total chlorophyll content were examined. To create salinity stress, NaCl was used in the experiment. The results revealed that different salinity stress had significant effects on all traits except for chlorophyll b and chlorophyll a/b. The cluster analysis in the control and at 75 and 150 mM salinity levels classified genotypes into two, two and three groups respectively. In each condition, the dpx and clean genotypes were placed in a group which the average traits were higher than the other genotypes. This can be generalized to the conditions of control as well as 75 and 150 mM salinity stress. Regression analysis showed possible informative loci encoding protein markers that was probable potential for selection strategies for salt weather proved by complementary tests.