Research regarding the myocytes thickness and profile of the lateral muscle in polyidon spathula species

Abstract

Histological samples from the lateral muscle (dorsal epaxial) were collected from ten, four year old, male sturgeon (PolyodonSpathula) specimens. These samples were processed in accordance with the paraffin sectioning method. Twenty histological slides were obtained with transversal sections from the above mentioned muscle. HEA coloration method was used and the slides were analyzed with a MC3 type optical microscope. Through microscopy, in the microscopic field, the small and big diameters of the primary muscle bundle (PMB) myocytes were determined. Calculations were made for the average diameter; perimeter and the values for the two indices that characterize the myocyte profile. The analysis was made on five secondary muscle bundles (SMB) and the obtained results were compared. The big myocyte diameter from the five SMB varied between48.397μ and52.605μ, with a mean of 51.363μ.Regarding the small myocyte diameter, it varied between 32.582μ and 37.119μ, with a mean of 35.238μ. The average thickness of the myocytes from the five SMB was between 40.489μ and 44.866μ, with a mean of 43.300μ. The average perimeter of the myocytes taken into this study was 136.048μ and the profile and shape index had average values of 70.303%, 1,512/1 respectively, which characterizes a cylindroid format with a tendency towards cylindrical. Most differences (95%) between the five SMB, for the 6 studied parameters are insignificant from a statistical point of view.