Determination of biofilm production in animals originated pseudomonas aeruginosa strains

Abstract

For the detection of Biofilm formation method, total 56 clinical isolates viz. Pseudomonas aeruginosa were used. Strains were identified using standards microbiological procedure. The susceptibility test of biofilm producing bacteria was performed by using disc diffusion technique. Biofilm detection was tested with Congo Red Agar method (CRA). These method is rapid, sensitive and reproducible, this method is suitable for detection of biofilm formation in the present study. Out of 56 isolates, CRA method detected 17 (30,35%) as high biofilm producer, and 39 (69,65 %) biofilm non- producer. According to the antibiotic susceptibility test, higher antibiotic resistance was observed in biofilm producing bacteria than non-biofilm producers.