The use of PCR for the detection of PRRS virus variants

Abstract

The etiologic agent is an ribovirus, initially called Lelystadt virus. Subsequent research has shown that this virus has two genotypes respectively, Type 1 (European) and type 2 (American) with an considerable variability of gene sequences, with an similarity of only 50-60%. For detection of this virus have been taken lymph nodes with pathological lesions, macroscopic characteristic forPRRS syndrome, from cadavers of youth porcine, from farms where this disease evolves. Finally were intended to RT-PCR technique, an number of 12 lymph nodes. By Real Time RT-PCR version, PRRS virus was detected in only one sample (0,08%) of the 12 samples examined. By the techniques recommended by GILBERT the virus was detected in all samples (100%) and by Nested RT-PCR techniques the virus was detected in five samples (41,66%). All positive samples provided by these techniques have demonstrated that the strains belong to type 1 (European).By using the technique recommended by TRUYEN, for the detection of type 2 (US) were not detected strains belonging to this type, all samples being negative