Abstract:
In order to investigate the
effect of exogenous application of nano-
TiO2 on annual medic, a field study was
conducted in a factorial design based on
randomized complete blocks with four
replications. The experimental treatments
included six concentrations of nano-TiO2
(Control, 0.01%, 0.02%, 0.03%, 0.04%,
0.06% g/l) and spraying at two growing
stages (pod stage and 10% flowering stage).
Results showed that the effects of nano-
TiO2 and spraying times on dry forage yield
were significant (p<0.01). Nano-TiO2 spray
appear to influence the malone dialdehyde
(MDA) content (p<0.01). With increasing
concentrations of nano-TiO2 the values of
aforementioned measured variable
significantly decreased. The activities of
antioxidant enzymes, including catalase
(CAT), ascorbate peroxidase (APX) and
guaiacol peroxidase (GPX) were affected by
nanoparticle (p<0.01) and spraying times
(p<0.01), as well as their interactive effect
of two mentioned factors were significant in
terms of guaiacol peroxidase (GPX)
(p<0.01) activity and dry forage yield.
Among different concentrations of nano-
TiO2, 0.04% and 0.06% have the best effect
on all traits. Totally, treatment with nano-
TiO2 were more effective in the pod stage,
compared to 10% flowering stage.
