Abstract:
In order to study the allelic
variation of Glu-1 gene (High molecular
weight glutenin), 100 Iranian wheat
cultivars including imported and domestic
genotypes were analyzed using ALP-PCR
technique. Four specific primer pairs were
used based on the genetic loci of Glu-A1,
Glu-B1, and Glu-D1 to perform the chain
polymerase reactions. PCR reaction
products were resolved on 2% agarose gel.
Since allele “a” had the largest relative
frequency (0.707), two alleles (a 344bp; b
362bp) were identified by P1-P2 primer for
Glu-A1 locus. Three alleles (a 800bp; b
500bp; c 300bp) were detected for Glu-B1
locus by P5-P6 primer, and allele “b” was
assumed as the highest relative frequency
(0.618). Two primer pairs were applied for
Glu-D1 locus. Ultimately, four alleles were
identified, where allele “c” had the highest
relative frequency (0.525). The observed
genetic variation value for Glu-D1 locus
(H=0.648) exhibits the maximal
polymorphism. Using cluster analysis, the
relationship between the observed
polymorphism and geographical variation
was investigated. The results indicated that
there exists a remarkable variation in Glu-
A1 locus between the Iranian wheat
cultivars.
