Research on the myocytes density and proportion of main tissue categories, from superficial pectoral muscles, of anas plathyrynchos species

Abstract

From a two years old female Anas plathyrynchos bird, common breed, weighing 1685 grams, we collected several histological samples from Pectoralis superficialis muscle (PS).Samples were processed by the paraffin sectioning technique yielding a few histological slides with the muscle cross sections mentioned above with HEA coloration. The sections have been studied on an MC3 optical microscope (OM), which previously has been adjusted and calibrated. In the microscopic field were made over 1200 myocytes micrometer measurements and 170 determinations of 17 primary muscle bundles (PMB) within 5 secundary muscle bundles (SMB/SMF).We determined: the average diameter; perimeter; profile and format index of the primary muscle fascicles (bundles). Also we determined the ratio of the myocytes density from muscle and connective tissue. We have obtained the following results: the average diameter of PMF of the 5 SMF ranged 285.936μ and 338.609μ, with an average value of 313.653μ. PMF average perimeter of the 5 SMF studied was 985.369μ , and for those two indicators that define the profile and format PMF (Fi= 1.8464 / 1; Pi = 62.0232 % ), the mean value of the 5 SMF, indicating a cylindroid shape with a flattening tendency (oval shape). The myocytes density varied between 460.831 and 596.577mf/square milimeters, with an average value of 525.933mf/mm².The proportion of muscle tissue was of 64.904 % and the proportion of 35.096 %. The differences between the average values of the five SMF studied for the 12 parameters analyzed, were found to be very statistically significant at a rate of 5% and not statistically significant in a proportion of 95 %.