Abstract:
Research on bovine oocytes
cryopreservation is important for successful
preservation of genetically valuable animal.
The transvaginal ultrasound-guided
follicular puncture coupled with in vitro
embryo production has become competitive
and alternative method for MOET (Multiple
ovulation and embryo transfer) in dairy
cattle. The aim of this preliminary research
is to presents the result of Bălțată cu Negru
Românească (BNR) cows oocytes recovery
by two different protocols and its
cryopreservation by slow freezing method.
By applying the recovery oocytes from
slaughterhouse ovary we obtained an
average of 16.34 ± 6.71 oocytes per cow,
much higher compared with the Ovum Pick
Up (OPU) method, which reveals an
average of 2.75 ± 0.2 oocytes per cow. After
applying the slow freezing procedures using
the ethylene glycol cryoprotectant we
observed the oocytes with cumulus cells
normal with the spherical shape and normal
zone pellucida.
