Evaluation of sows oocytes viability through Trypan Blue staining after vitrification

Abstract

Along sperm and embryo cryopreservation that are used routinely also in animal assisted reproduction, studying are done also on animal oocyte cryopreservation in order to find the best conditions to preserve their viability. Vitrification is one of the methods that can be used in order to preserve oocytes. The higher reactive oxygen species that are formed during in vitro conditions can influence the success of assisted reproduction technique. The aim of this study was to evaluate the antioxidant potential of ascorbic acid (0.5mM) and rosmarinic acid (105μM) added in media for in vitro maturation on swine oocyte subjected to vitrification. COC’s viability after vitrification was evaluated by 0.02% Trypan Blue staining. Comparing experimental groups C (vitamin C) and AR (rosmarinic acid) with group M (control), relative to the number of vitrified oocytes, a slight increase in their viability is observed, with 16.67% (C, class I) and 33.33% (AR, class II), respectively. Regardless of the treatment applied, the oocyte class is associated with viability (p = 0.048). Due to low number of oocytes used in each group we can concluded that supplementation of oocyte maturation media before vitrification with rosmarinic acid and ascorbic acid could produce a slight increase in viability.