Effect of rozmarinic acid supplementation on in vitro maturation of bovine oocytes

Abstract

Antioxidants supplementation of in vitro culture media exerts the key role to reduce the effects of reactive oxidative species produced during assisted reproduction technique. The objective of the study was to determine the effect of rosmarinic acid addition to the in vitro culture media on bovine oocytes maturation rate based on morphological changes. Bovine COC’s were matured according to their morphological class (class I, II and III) in two groups: control (M) and supplemented with rosmarinic acid (105 μM, AR) in TCM 199 HEPES modification media at 38.50C in 5% CO2 humidified air atmosphere for 24h. Comparing the groups, relative to the number of COC’s matured, a increase in their maturation features is observed, with 26.81% % (AR1), 21.67% (AR2) and 23.34% (AR3), respectively in groups supplemented with rosmarinic acid. The oocyte class is associated with their capacity to develop in vitro based on their morphological examination.