Determination of protein fractions in a sports horse with laryngotracheitis, nonspecifically stimulated with a phytotherapeutic extract (case study

Abstract

In a sports horse with signs of laryngotracheitis that hardly achieved the training program, a phytotherapeutic extract with immunomodulating properties was administered. Following the administration of the extract, on the second day, the horse showed a remarkable comeback in the sense that it did the normal training without the presence of fatigue and coughing. The horse was clinically examined, blood samples were collected and the extract was inoculated s.c., blood samples were collected at 24 hours, 7 days and 10 days for haematological, biochemical examinations and also for electrophoresis, in order to determin the protein fractions. Significant changes are noted for fractions β1, β2 and γ. In terms of albumin, they are found initially at physiological values, and 24 hours after the inoculation of the extract, very significant decreased throughout the experiment. The most spectacular variations of the protein fractions are those for gamma globulins: initially they are drastically decreased, and 24 hours after the inoculation of the extract, they increased spectacularly, about 3 times, and remain close to this level (in physiological parameters), throughout the experiment. The albumin / globulin ratio is clearly in favor of globulins.