Clostridium perfringens enterotoxigen involved in hemorrhagic diarrhea at dogs

Abstract

Clostridium perfringens is a commensal of the human and animal intestines. The toxigenic strains of this bacterial species are responsible for some enteral diseases in humans and domestic animals. In dogs, hemorrhagic enteritis produced by Clostridium perfringens are sporadic but have a severe progression-restricted progression. In the year 2018, 9 faeces samples were taken from dewormed dogs, vaccinated but with enteritis and associated toxic conditions (vomiting, lethargy, diminished appetite) that started suddenly without any other clinical history. Based on the anamnesis, it excluded the risk of chemical poisoning. Faeces samples were subjected to the microbiological exam. The bacterioscopic examination in Gram stained smears was predominantly Gram positive bacilli with morphology and characteristic disposition for Clostridium sp and no specific morphological phenotypes for spirochetes or protozoa were identified. The samples were incubated at 37° C, under anaerobic conditions, on liquid culture media (bullion VL) and solids (Clostridium agar, SPS agar) and under aerobic conditions on culture media for aerobic bacteria (nutrient broth, blood agar) (E. coli, Salmonella sp., Shigella sp., Campylobacter sp., Yersinia sp., Serpulina sp., Vibrio sp., etc.) which can trigger enteritis in dogs. Following the bacteriological examination, strains of Clostridium sp. Biochemical tests have included the species as Clostridium perfrigens. The clinical progression in the 9 patients was different: 6 dogs responded to antibiotic therapy, recommended on the basis of the antibiotic, and 3 dogs died within 48 hours before a treatment was instituted.