Evaluation of antifungal susceptibility of some Candida SPP. Strains using Multodisc antifungal disk system

Abstract

The aim of this study was to evaluate the antifungal susceptibility of 33 Candida spp. isolates to classic antifungals included in Multodisc® system kits (Liofilchem – Italy). The research was conducted during January-June 2014 in the Department of Microbiology, Faculty of Veterinary Medicine, Cluj-Napoca, Romania. Identification was made from 48 hrs isolated colonies, using microscopic and biochemical methods (API Candida). From the total of 33 tested strains, 13 were represented by Candida albicans, 9 strains were identified as Candida krusei, 6 were Candida tropicalis 3 Candida catenulata and 2 were Candida parapsilosis. The antifungals included in the disk were represented by Econazole, Nystatin, Griseofulvin, Amphotericin B, Flucytosine, Miconazole, Metronidazole and Ketoconazole. The susceptibility test principle was based on the disk diffusion test, while the interpretation was performed determining the inhibition area diameter. The most efficient antifungal was represented by Ketoconazole with an average of the inhibition area of 26.4 mm followed by Miconazole with 23.4 mm and Econazole with 22.7 mm. The least efficient antifungals were represented by Metronidazole, Griseofulvin and Nystatin.