Abstract:
The analysis of biochemical blood indices is essential for testing the bioequivalence and drug
bioavailability. The purpose of this study is to analyze the relevance of the metabolic profile as an indicator of health on
a sheep sample and the bioequivalence testing of two anthelmintic products in view of interchanging them in therapeutic
practice. The research required a sample of 36 clinically healthy sheep. The sheep were selected on the premise of their
health parameters being within the physiological, clinical and hematological indexes. One unicentric, randomized,
cross-over with two-stage, two treatments and a pause were used in the test, including: initial screening and animal
selection, phase I, washout, phase II, and final screening. Determination of blood biochemical indexes was performed
during initial and final screening and consisted of blood samples (on EDTA) by automatic method, with VetScan analyzer
and Large Animal Profile kits, including: ALP-alkaline phosphatase (U / L), AST (U / L), GGT-gammaglutamyltransferase
(U / L), BUN-ureic nitrogen in the blood (mmol / L), total protein (g / L), GLOB-globulins (g / L),
Ca-calcium (mmol / L), P-phosphorus (mmol / L), Mg - Magnesium (mmol / L). The results obtained revealed significant
oscillations of most of the biochemical blood indices, but within the physiological range of the species and age, with
some exceptions. Deviations consisted of ALP, CK and albumin slightly rising above the upper physiological limits,
which we associated with stress-induced manipulation. Considering that the physical effort intensifies the CK activity,
we attribute the higher values recorded in some sheep to the muscular effort generated by the natural grazing and the
manipulation during the test. On the other hand, the slight increases in albuminemia correlated with dehydration. The
integration of the values obtained in the evaluation of the biochemical blood indexes in the results of the clinical,
haematological and statistical parameters was the confirmation that the sheep's health was in accordance with the
requirements imposed by the protocol elaborated for the bioequivalence testing of the anthelmintic products tested.
